Venom of Synthetic derivative of Bungarus multicinctus toxin
| Product ID | PTX-PPT-009 |
|---|---|
| Name | Synthetic derivative of Bungarus multicinctus toxin |
| Common Name(s) | alpha bungarotoxin, nicotinic receptor imaging, fluorescent toxin, nAChR probe |
| Origin | Solid-phase synthesis with site-selective fluorophore conjugation |
| Purity | >= 95% by HPLC |
| Form | Lyophilized peptide, 50 μg per vial |
| Packaging | In vacuum sealed glass vials, in secured parcel. |
| Packaging | Price ($) | Quantity | Buy Now |
|---|---|---|---|
| 100 mg | 900.00 |
| IUCN Status | Not Evaluated |
|---|---|
| CITES | See CITES Appendices |
| Local | Check Local Regulations |
Description & Applications
Description & origin
Venom Supplies synthesizes this α-bungarotoxin analog with a site-directed lysine-to-azidoalanine substitution enabling click chemistry conjugation to a far-red fluorophore (λex 640 nm, λem 660 nm). Purification ensures removal of unconjugated dye, and analytical HPLC confirms > 95% purity. Mass spectrometry validates conjugate mass and disulfide integrity.
Molecular targets
The derivative retains high-affinity binding to α7 and muscle-type nicotinic acetylcholine receptors (Kd 1.2 nM and 1.8 nM, respectively). Fluorescence lifetime data confirm compatibility with confocal and super-resolution microscopy. COA documentation supplies spectral properties, quantum yield, and photostability metrics.
Research applications
- Receptor imaging: Visualize nAChR distribution in live neurons or neuromuscular junctions.
- Synaptic labeling: Combine with electrophysiology to track receptor trafficking.
- Ligand binding assays: Use in fluorescence polarization or FRET assays for screening.
Link to Peptide Toxins, Neuroscience application, and blog resource From venom to therapeutic lead: an overview for researchers.
Format & handling
Reconstitute with 50 μL PBS containing 2 mM sodium azide to preserve sterility. Protect from light and store in amber vials. Avoid repeated freeze–thaw cycles; prepare working dilutions fresh.
Documentation
- COA: purity profile, fluorescence spectra, binding constants, endotoxin (< 0.05 EU/μg).
- SDS: hazard statements, PPE guidance, spill procedures.
Safety notes
Work within a light-controlled biosafety cabinet. Wear double gloves and protective eyewear. Decontaminate surfaces with 0.5% sodium hypochlorite.
Related resources
- Companion product: PTX-RCT-001 Recombinant α-Bungarotoxin
- Application synergy: Receptor Pharmacology
- Technical assistance: Support
Synthetic derivative of Bungarus multicinctus toxin