Venom of Recombinant analog of Conus magus peptide
| Product ID | PTX-RCT-004 |
|---|---|
| Name | Recombinant analog of Conus magus peptide |
| Common Name(s) | omega conotoxin mviia, recombinant cone snail toxin, cav2.2 inhibitor, analgesic reference |
| Origin | Pichia pastoris fermentation with oxidative folding |
| Purity | >= 96% by HPLC |
| Form | Lyophilized peptide, 100 μg per vial |
| Packaging | In vacuum sealed glass vials, in secured parcel. |
| Packaging | Price ($) | Quantity | Buy Now |
|---|---|---|---|
| 100 mg | 950.00 |
| IUCN Status | Not Evaluated |
|---|---|
| CITES | See CITES Appendices |
| Local | Check Local Regulations |
Description & Applications
Description & origin
The recombinant ω-conotoxin MVIIA is expressed in Pichia pastoris with secretory signal peptides enabling oxidative folding in the endoplasmic reticulum. Post-fermentation, the supernatant undergoes cation exchange and RP-HPLC purification. Final quality control includes mass spectrometry, analytical HPLC, and circular dichroism to confirm native-like folding.
Molecular targets
The peptide exhibits IC₅₀ 0.85 nM at CaV2.2 and 4.5 nM at CaV2.1. Whole-cell voltage-clamp assays on dorsal root ganglion neurons demonstrate > 90% reduction in high-voltage activated currents at 100 nM. The COA supplies inhibition curves and suggested protocols for cumulative dosing experiments.
Research applications
- Pain therapeutic benchmarking: Compare investigational inhibitors against a clinically validated toxin scaffold.
- Synaptic transmission studies: Quantify toxin-induced suppression of neurotransmitter release.
- Calcium channel pharmacology: Explore state dependence and recovery kinetics in neuronal preparations.
Crosslinks: Recombinant Toxins category, Pain Research, and blog reference How venom-derived toxins advance ion channel research.
Format & handling
Reconstitute with 100 μL 20 mM HEPES, 150 mM NaCl, pH 7.4. Aliquot into sterile, low-binding tubes and store at −80 °C. Avoid multiple freeze–thaw cycles.
Documentation
- COA: purity, mass spectrum, potency data, endotoxin (< 0.05 EU/μg).
- SDS: hazard overview, PPE, emergency response instructions.
Safety notes
Implement BSL-2 containment, including double gloves and splash protection. Decontaminate surfaces with oxidizing solutions.
Related resources
- Companion product: PTX-MTX-001 ω-Conotoxin CVIF
- Application synergy: Ion Channel Research
- Technical assistance: Support
Recombinant analog of Conus magus peptide