Venom of Phoneutria nigriventer
| Product ID | PTX-SPV-002 |
|---|---|
| Name | Phoneutria nigriventer |
| Common Name(s) | phoneutria venom, nav1.7 toxin, pain research peptide, spider toxin |
| Origin | Captive-bred specimens maintained in controlled humidity facilities |
| Purity | >= 88% accumulated peptide content |
| Form | Lyophilized fraction, 200 μg per vial |
| Packaging | In vacuum sealed glass vials, in secured parcel. |
| Packaging | Price ($) | Quantity | Buy Now |
|---|---|---|---|
| 100 mg | 700.00 |
| IUCN Status | Not Evaluated |
|---|---|
| CITES | See CITES Appendices |
| Local | Check Local Regulations |
Description & Applications
Description & origin
This product pools chromatographically separated peptide fractions from Phoneutria nigriventer venom, emphasizing Pn3a and related NaV-active molecules. The venom undergoes ultrafiltration and preparative RP-HPLC to isolate 3–5 kDa peptides, ensuring minimal contamination by enzymatic components. Mass spectrometry verifies molecular masses and disulfide connectivity consistent with inhibitor cystine knot motifs.
Molecular targets
Whole-cell patch-clamp data indicate IC₅₀ values of 4.6 nM for NaV1.7, 9.3 nM for NaV1.8, and 12.1 nM for NaV1.9, with negligible activity on cardiac NaV1.5. Inhibition is state-dependent, stabilizing channels in the closed configuration and slowing recovery from inactivation. COA supplements feature gating shift plots and protocol parameters.
Research applications
- Pain target validation: Benchmark analgesic candidates targeting NaV1.7 or NaV1.9.
- Sensory neuron excitability profiling: Evaluate dorsal root ganglion firing in the presence of defined blockers.
- Drug screening reference: Calibrate automated patch-clamp platforms for NaV potency ranking.
Explore the broader Spider Venoms category and align experiments with the Pain Research application guide. For deeper mechanistic insight, consult the blog analysis How venom-derived toxins advance ion channel research.
Format & handling
Reconstitute 200 μg in 200 μL nuclease-free water containing 0.1% acetonitrile and 0.1% formic acid. Aliquot immediately, flash-freeze in liquid nitrogen, and store at −80 °C to preserve activity.
Documentation
- COA: peptide composition, activity curves, endotoxin (< 0.1 EU/μg), residual salt analysis.
- SDS: hazard communication, PPE recommendations, first-aid measures.
Safety notes
Use respiratory protection if working outside a certified biosafety cabinet. Implement dedicated waste containers for peptide solutions and decontaminate with oxidizing agents.
Related resources
- Complementary product: PTX-PPT-002 μ-Conotoxin Analog
- Application synergy: Neuroscience
- Technical consultation: Support
Phoneutria nigriventer